Hotspot

ndhC-trnV-UAC

LSC · 49675-50635 · 961 bp

Back to Paraleonurus

Pi 0.00166

MarkerSeek score 52.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 4
pi: 0.00166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1092 bp	1.0	78.1
ndhC-trnV-UAC_p2	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1113 bp	1.0	75.4
ndhC-trnV-UAC_p3	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1091 bp	1.0	75.3
ndhC-trnV-UAC_p4	TCTCCGGCCCTTTCCTAATA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1112 bp	1.0	74.9
ndhC-trnV-UAC_p5	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1080 bp	1.0	74.2