Hotspot

petN-psbM

LSC · 28798-29581 · 784 bp

Back to Paraleonurus

Pi 0.00561

MarkerSeek score 65.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 10
pi: 0.00561
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	861-870 bp	1.0	78.7
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	859-868 bp	1.0	78.7
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TCCTACCGCCTTTCTGCTTA	55.0	50.0	57.9	58.4	858-867 bp	1.0	78.1
petN-psbM_p4	ACTCGTAGTGTGGGGAAGAA	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	860-869 bp	1.0	77.2
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	858-867 bp	1.0	77.2