Hotspot

atpH-atpI

LSC · 13451-14378 · 928 bp

Back to Paraleonurus

Pi 0.00259

MarkerSeek score 60.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 6
pi: 0.00259
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1062-1068 bp	1.0	79.1
atpH-atpI_p2	TCCGATAGAAGCAAGCCCTA	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	1099-1105 bp	1.0	78.7
atpH-atpI_p3	TCCAGGTCCGATAGAAGCAA	CCCGTCATGTTCCTTGGATT	50.0	50.0	58.1	57.9	1105-1111 bp	1.0	78.3
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	57.9	57.9	1078-1084 bp	1.0	78.2
atpH-atpI_p5	CCGATAGAAGCAAGCCCTAC	CCCGTCATGTTCCTTGGATT	55.0	50.0	57.8	57.9	1098-1104 bp	1.0	78.2