Hotspot

atpF-atpH

LSC · 12835-13204 · 370 bp

Back to Paraleonurus

Pi 0.00216

MarkerSeek score 53.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 2
pi: 0.00216
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	CGGAGGGAAAAATACGAGGT	45.0	50.0	57.9	57.3	512-521 bp	1.0	76.8
atpF-atpH_p2	TGGCCCAAAGAAACGAAAGA	GAGGCGGAGGGAAAAATACG	45.0	55.0	57.9	58.7	516-525 bp	1.0	76.7
atpF-atpH_p3	TGGCCCAAAGAAACGAAAGA	GCGGAGGGAAAAATACGAGG	45.0	55.0	57.9	58.7	513-522 bp	1.0	76.7
atpF-atpH_p4	TGGCCCAAAGAAACGAAAGA	GGCGGAGGGAAAAATACGAG	45.0	55.0	57.9	58.7	514-523 bp	1.0	76.7
atpF-atpH_p5	TGGCCCAAAGAAACGAAAGA	CGAGGCGGAGGGAAAAATAC	45.0	55.0	57.9	58.7	517-526 bp	1.0	76.6