Hotspot

psbI-trnG-UCC

LSC · 8012-8908 · 897 bp

Back to Paraleonurus

Pi 0.00334

MarkerSeek score 42.00

Primer available yes

Label psbI-trnG-UCC

Feature Metadata

label_name: psbI-trnG-UCC
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 7
pi: 0.00334
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnG-UCC_p1	AATGATCCAGGGCGAAATCC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	967-970 bp	1.0	52.2
psbI-trnG-UCC_p2	AATGATCCAGGGCGAAATCC	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	970-973 bp	1.0	51.1
psbI-trnG-UCC_p3	AATGATCCAGGGCGAAATCC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	969-972 bp	1.0	51.1
psbI-trnG-UCC_p4	AATGATCCAGGGCGAAATCC	AACGAATCACACTTTTACCACT	50.0	36.4	58.0	56.2	968-971 bp	1.0	50.3
psbI-trnG-UCC_p5	CGAAATCCTGGACGTGAAGA	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	955-958 bp	1.0	49.4