Hotspot

rpl22

LSC · 84493-85122 · 630 bp

Back to Parakeelya

Pi 0.174

MarkerSeek score 85.10

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 630
variable_sites: 102
pi: 0.174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAAGTCGGAAACCAAGTGGA	TGTTCATTAATGGGAGGTAAACT	50.0	34.8	58.0	55.6	633-676 bp	1.0	52.0
rpl22_p2	ACCAAGTCGGAAACCAAGTG	TGTTCATTAATGGGAGGTAAACT	50.0	34.8	58.3	55.6	635-678 bp	1.0	52.0
rpl22_p3	CAAGTCGGAAACCAAGTGGA	TGTTCATTAATGGGAGGTAAACTT	50.0	33.3	58.0	56.3	633-676 bp	1.0	52.0
rpl22_p4	CAAGTCGGAAACCAAGTGGA	TTGTTCATTAATGGGAGGTAAACT	50.0	33.3	58.0	56.3	634-677 bp	1.0	52.0
rpl22_p5	CAAGTCGGAAACCAAGTGGA	TGTTCATTAATGGGAGGTAAAC	50.0	36.4	58.0	54.1	633-676 bp	1.0	52.0