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psbE-petL

LSC · 65302-66480 · 1179 bp

Back to Parakeelya

Pi 0.052

MarkerSeek score 83.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 61
pi: 0.052
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAAAAACCCG	50.0	50.0	57.9	58.6	1326-1335 bp	1.0	79.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCGCTAGTAAAAACCCGAA	50.0	50.0	57.9	58.6	1324-1333 bp	1.0	79.2
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAAAAACCCG	50.0	50.0	57.8	58.6	1249-1258 bp	1.0	78.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAAAAACCCGAA	50.0	50.0	57.8	58.6	1247-1256 bp	1.0	78.6
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAAAAACCCG	45.0	50.0	58.2	58.6	1335-1344 bp	1.0	78.3