Hotspot

ycf3-trnS-GGA

LSC · 44391-44842 · 452 bp

Back to Parakeelya

Pi 0.0629

MarkerSeek score 81.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 27
pi: 0.0629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	541-759 bp	1.0	77.1
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	512-730 bp	1.0	76.6
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	562-780 bp	1.0	76.4
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	514-732 bp	1.0	76.0
ycf3-trnS-GGA_p5	TGGCCACAATTGAAAAGGTC	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.8	58.1	577-795 bp	1.0	75.5