Hotspot

psaA-ycf3

LSC · 41484-42369 · 886 bp

Back to Parakeelya

Pi 0.0627

MarkerSeek score 82.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 886
variable_sites: 55
pi: 0.0627
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGTTTGGCCCATTCCTCAAA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.7	58.0	1070-1115 bp	1.0	79.1
psaA-ycf3_p2	TTTTGACTTCTGGTTCCGGC	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.7	58.0	1018-1063 bp	1.0	79.1
psaA-ycf3_p3	TGGCCCATTCCTCAAAAGAA	ATCAAGCCGCTGAGTATTGG	45.0	50.0	57.3	58.0	1066-1111 bp	1.0	78.8
psaA-ycf3_p4	TTGGCCCATTCCTCAAAAGA	ATCAAGCCGCTGAGTATTGG	45.0	50.0	57.3	58.0	1067-1112 bp	1.0	78.8
psaA-ycf3_p5	GGTTTGGCCCATTCCTCAAA	CGATAACGCTTACTCCTGGG	50.0	55.0	58.7	57.9	1043-1088 bp	1.0	78.5