Hotspot

psbM-trnD-GUC

LSC · 29049-29780 · 732 bp

Back to Parakeelya

Pi 0.0755

MarkerSeek score 82.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 55
pi: 0.0755
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.2	58.5	843-1312 bp	1.0	58.0
psbM-trnD-GUC_p2	ACTAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.0	58.5	846-1315 bp	1.0	56.5
psbM-trnD-GUC_p3	AGGAACTAGAATGAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	43.5	50.0	57.9	58.5	850-1319 bp	1.0	55.9
psbM-trnD-GUC_p4	AGGAACTAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	56.8	58.5	850-1319 bp	1.0	55.2
psbM-trnD-GUC_p5	GGAACTAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.2	58.5	849-1318 bp	1.0	55.2