Hotspot

trnS-GCU-trnG-UCC

LSC · 7999-8811 · 813 bp

Back to Parakeelya

Pi 0.0903

MarkerSeek score 82.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 68
pi: 0.0903
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTCGTCCACTCAGCCATC	GGGTTAGTAGAACGAATCGCA	55.0	47.6	58.6	57.8	834-883 bp	1.0	76.0
trnS-GCU-trnG-UCC_p2	CTTTCGTCCACTCAGCCATC	ACGAATCGCACTTTTACCACT	55.0	42.9	58.6	58.2	823-872 bp	1.0	75.4
trnS-GCU-trnG-UCC_p3	CTTTCGTCCACTCAGCCATC	ACGAATCGCACTTTTACCAC	55.0	45.0	58.6	56.8	823-872 bp	1.0	75.4
trnS-GCU-trnG-UCC_p4	CTTTCGTCCACTCAGCCATC	AACGAATCGCACTTTTACCAC	55.0	42.9	58.6	57.5	824-873 bp	1.0	73.1
trnS-GCU-trnG-UCC_p5	CTTTCGTCCACTCAGCCATC	GGGGTTAGTAGAACGAATCGC	55.0	52.4	58.6	58.5	835-884 bp	1.0	73.0