Hotspot

psaB

LSC · 36704-38908 · 2205 bp

Back to Paragynoxys

Pi 0.00227

MarkerSeek score 58.60

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 5
pi: 0.00227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	GGCGGATCTCTTCTTCATCG	ACAACATGGGCGTTCTTCTT	55.0	45.0	58.2	58.0	2340 bp	1.0	78.2
psaB_p2	GGCGGATCTCTTCTTCATCG	TACAAGGACGTGCTGTAGGA	55.0	50.0	58.2	58.1	2390 bp	1.0	77.7
psaB_p3	GGCGGATCTCTTCTTCATCG	GGGCGTTCTTCTTAGCAAGA	55.0	50.0	58.2	57.9	2333 bp	1.0	77.6
psaB_p4	GGCGGATCTCTTCTTCATCG	TGGGCGTTCTTCTTAGCAAG	55.0	50.0	58.2	58.2	2334 bp	1.0	76.9
psaB_p5	GGCGGATCTCTTCTTCATCG	AGGACGTGCTGTAGGAGTAA	55.0	50.0	58.2	57.8	2386 bp	1.0	76.7