Hotspot

petB

LSC · 73869-75278 · 1410 bp

Back to Paragynoxys

Pi 0.00213

MarkerSeek score 60.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1410
variable_sites: 3
pi: 0.00213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CCCCCAAGTCCTAGCTTTTC	ATCGTTCCTACCCCAGTGAT	55.0	50.0	57.9	57.8	1587 bp	1.0	77.7
petB_p2	TTTTGGTAGTTCGACCGTGG	ATCGTTCCTACCCCAGTGAT	50.0	50.0	58.1	57.8	1522 bp	1.0	77.7
petB_p3	GGTAGTTCGACCGTGGAATT	ATCGTTCCTACCCCAGTGAT	50.0	50.0	57.6	57.8	1518 bp	1.0	75.7
petB_p4	AACCCCCAAGTCCTAGCTTT	ATCGTTCCTACCCCAGTGAT	50.0	50.0	58.5	57.8	1589 bp	1.0	74.4
petB_p5	ACCCCCAAGTCCTAGCTTTT	ATCGTTCCTACCCCAGTGAT	50.0	50.0	58.5	57.8	1588 bp	1.0	74.4