Hotspot

ndhB

IRa · 138791-140994 · 2204 bp

Back to Paragynoxys

Pi 0.000908

MarkerSeek score 43.60

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: IRa
length_bp: 2204
variable_sites: 2
pi: 0.000908
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ATGGCAGAGGCAAATAGAGC	TTACCATTTTGGGCGGAACA	50.0	45.0	58.0	58.0	2617 bp	1.0	80.0
ndhB_p2	TTGGTTATTAGCGGCATCCC	TTACCATTTTGGGCGGAACA	50.0	45.0	58.0	58.0	2741 bp	1.0	79.9
ndhB_p3	ATGGCAGAGGCAAATAGAGC	GGACATCCAACTGCTTCGAT	50.0	50.0	58.0	58.0	2984-2986 bp	1.0	79.9
ndhB_p4	TCCGAATTAGTGGATGCTGC	TTACCATTTTGGGCGGAACA	50.0	45.0	58.0	58.0	2683 bp	1.0	79.8
ndhB_p5	GGCAGAGGCAAATAGAGCTT	TTACCATTTTGGGCGGAACA	50.0	45.0	58.0	58.0	2615 bp	1.0	79.7