Hotspot

accD

LSC · 56610-58106 · 1497 bp

Back to Paragynoxys

Pi 0.002

MarkerSeek score 59.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 3
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTTTCAATACTCACACTCCTT	GTCAACAAAGACAACCCTATTCG	33.3	43.5	52.7	58.0	1624 bp	1.0	40.1
accD_p2	TTTTTCAATACTCACACTCCT	GTCAACAAAGACAACCCTATTCG	33.3	43.5	52.7	58.0	1625 bp	1.0	40.1
accD_p3	TTTTCAATACTCACACTCCTT	CAACAAAGACAACCCTATTCGT	33.3	40.9	52.7	56.9	1622 bp	1.0	40.1
accD_p4	TTTTTCAATACTCACACTCCT	CAACAAAGACAACCCTATTCGT	33.3	40.9	52.7	56.9	1623 bp	1.0	40.1
accD_p5	TTTTTCAATACTCACACTCCTT	GTCAACAAAGACAACCCTATTCG	31.8	43.5	53.6	58.0	1625 bp	1.0	40.1