Hotspot

rpl32-ndhF

SSC · 122857-123918 · 1062 bp

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Pi 0.00284

MarkerSeek score 60.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1062
variable_sites: 3
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACAAAGCTTTTAACGCTGCC	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1254-1257 bp	1.0	79.1
rpl32-ndhF_p2	AACGCTGCCCAATATCCTTT	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1243-1246 bp	1.0	79.0
rpl32-ndhF_p3	ACAAAGCTTTTAACGCTGCC	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	1252-1255 bp	1.0	79.0
rpl32-ndhF_p4	ACAAAGCTTTTAACGCTGCC	TTGCCGTCGGAAAAAGAAGA	45.0	45.0	57.9	58.0	1253-1256 bp	1.0	79.0
rpl32-ndhF_p5	ACAAAGCTTTTAACGCTGCC	TACTTTTTGTTGCCGTCGGA	45.0	45.0	57.9	58.0	1262-1265 bp	1.0	79.0