Hotspot

trnL-CAA-ndhB

IRb · 92597-93170 · 574 bp

Back to Paragynoxys

Pi 0.0139

MarkerSeek score 53.80

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 574
variable_sites: 8
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	634-636 bp	1.0	80.5
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	632-634 bp	1.0	80.5
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.1	57.9	622-624 bp	1.0	79.9
trnL-CAA-ndhB_p4	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	629-631 bp	1.0	79.0
trnL-CAA-ndhB_p5	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	633-635 bp	1.0	78.4