Hotspot

rpl36-infA

LSC · 78595-78709 · 115 bp

Back to Paragynoxys

Pi 0.0174
MarkerSeek score 62.50
Primer available yes
Label rpl36-infA

Feature Metadata

label_name
rpl36-infA
feature_type
igs
region
LSC
length_bp
115
variable_sites
2
pi
0.0174
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-infA_p1 GTTTATGTCTTGGGTTGGAACA ACGGATACTGCCAGGAGATA 40.9 50.0 56.8 57.3 318 bp 1.0 49.5
rpl36-infA_p2 GTTTATGTCTTGGGTTGGAACA ATACGGATACTGCCAGGAGA 40.9 50.0 56.8 57.3 320 bp 1.0 49.5
rpl36-infA_p3 GTTTATGTCTTGGGTTGGAACA TACGGATACTGCCAGGAGAT 40.9 50.0 56.8 57.3 319 bp 1.0 49.5
rpl36-infA_p4 TGTTTATGTCTTGGGTTGGAAC ACGGATACTGCCAGGAGATA 40.9 50.0 56.8 57.3 319 bp 1.0 49.5
rpl36-infA_p5 TGTTTATGTCTTGGGTTGGAAC TACGGATACTGCCAGGAGAT 40.9 50.0 56.8 57.3 320 bp 1.0 49.5