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psbE-petL

LSC · 63670-64900 · 1231 bp

Back to Paragynoxys

Pi 0.00509

MarkerSeek score 65.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1231
variable_sites: 6
pi: 0.00509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1377-1423 bp	1.0	75.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1300-1346 bp	1.0	74.4
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1386-1432 bp	1.0	74.1
psbE-petL_p4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1391-1437 bp	1.0	73.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1385-1431 bp	1.0	73.9