Hotspot

atpB-rbcL

LSC · 53895-54630 · 736 bp

Back to Paragynoxys

Pi 0.00272

MarkerSeek score 60.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 2
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGCCCCAGGTTTGTTTT	GCTTTGAATCCAACACTCGC	45.0	50.0	58.0	58.0	848-849 bp	1.0	79.9
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	GCTTTGAATCCAACACTCGC	50.0	50.0	58.0	58.0	892-893 bp	1.0	79.7
atpB-rbcL_p3	CGTGGTAACCGCAGAACTAG	GCTTTGAATCCAACACTCGC	55.0	50.0	58.4	58.0	822-823 bp	1.0	77.0
atpB-rbcL_p4	GATACGCCCCAGGTTTGTTT	GCTTTGAATCCAACACTCGC	50.0	50.0	58.5	58.0	849-850 bp	1.0	76.3
atpB-rbcL_p5	CATTTTGCCTGGCGGAAAAG	GCTTTGAATCCAACACTCGC	50.0	50.0	58.6	58.0	900-901 bp	1.0	75.4