Hotspot

petN-psbM

LSC · 10425-10924 · 500 bp

Back to Paragynoxys

Pi 0.00402

MarkerSeek score 47.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 2
pi: 0.00402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGAAGTGGACT	TGCTACTGCACTGTTCATTT	47.6	40.0	57.8	55.6	604 bp	1.0	51.2
petN-psbM_p2	AGTATGGGGAAGAAGTGGACT	TGCTACTGCACTGTTCATTTT	47.6	38.1	57.8	56.4	604 bp	1.0	49.5
petN-psbM_p3	CCCTTTCACTCGTAGTATGGG	TGCTACTGCACTGTTCATTT	52.4	40.0	57.5	55.6	617 bp	1.0	49.2
petN-psbM_p4	CCTTTCACTCGTAGTATGGGG	TGCTACTGCACTGTTCATTT	52.4	40.0	57.5	55.6	616 bp	1.0	49.2
petN-psbM_p5	CGTAGTATGGGGAAGAAGTGG	TGCTACTGCACTGTTCATTT	52.4	40.0	57.5	55.6	607 bp	1.0	49.2