Hotspot

rps16

LSC · 5242-6345 · 1104 bp

Back to Paracalia

Pi 0.00726

MarkerSeek score 57.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1104
variable_sites: 8
pi: 0.00726
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAATAGAGCAAGAAAAGAAGGA	GTCTATGTACCGAGCGAACC	34.8	55.0	55.6	57.9	1302-1307 bp	1.0	40.6
rps16_p2	AGAGCAAGAAAAGAAGGAAA	GTCTATGTACCGAGCGAACC	35.0	55.0	52.6	57.9	1297-1302 bp	1.0	40.6
rps16_p3	TGAATAGAGCAAGAAAAGAAGGA	CCGAGCGAACCAATGACTAT	34.8	50.0	55.6	57.8	1293-1298 bp	1.0	40.6
rps16_p4	AGAGCAAGAAAAGAAGGAAA	CCGAGCGAACCAATGACTAT	35.0	50.0	52.6	57.8	1288-1293 bp	1.0	40.6
rps16_p5	TGAATAGAGCAAGAAAAGAAGGA	TCTATGTACCGAGCGAACCA	34.8	50.0	55.6	58.2	1301-1306 bp	1.0	40.6