Hotspot

rpoC1

LSC · 16136-18956 · 2821 bp

Back to Paracalia

Pi 0.00532

MarkerSeek score 59.10

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2821
variable_sites: 15
pi: 0.00532
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCTGAGAAGACTTTCCAGCT	GTACCTCCATATTGCGCTGA	45.0	50.0	55.8	57.5	2976-2980 bp	1.0	58.6
rpoC1_p2	TCTGAGAAGACTTTCCAGCT	TCAGTACCTCCATATTGCGC	45.0	50.0	55.8	57.5	2979-2983 bp	1.0	58.6
rpoC1_p3	TTCGGTTGTTCGTTCGAGAA	GGGTTTGAGTCAGTGATTGGA	45.0	47.6	57.8	57.9	3000 bp	0.5	49.2
rpoC1_p4	TTCGGTTGTTCGTTCGAGAA	GGGTTTGAGTCAGTGATTGG	45.0	50.0	57.8	56.3	3000 bp	0.5	45.0
rpoC1_p5	TTCGGTTGTTCGTTCGAGAA	GGGTTTGAGTCAGTGATTGGAA	45.0	45.5	57.8	58.5	3000 bp	0.5	38.2