Hotspot

rpoB

LSC · 12948-16130 · 3183 bp

Back to Paracalia

Pi 0.0066

MarkerSeek score 45.30

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3183
variable_sites: 21
pi: 0.0066
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	CCGAACACGAGGGAAAGATT	GCTCGCTAATGAGTACTGCA	50.0	50.0	57.9	57.8	285 bp	1.0	79.0
rpoB_p2	AGATTCAGGGGCTCTTGCTA	GCTCGCTAATGAGTACTGCA	50.0	50.0	58.1	57.8	308 bp	1.0	78.7
rpoB_p3	TAGATTCAGGGGCTCTTGCT	GCTCGCTAATGAGTACTGCA	50.0	50.0	58.1	57.8	309 bp	1.0	78.7
rpoB_p4	CAAGCGGCTCTAGATTCAGG	GCTCGCTAATGAGTACTGCA	55.0	50.0	58.1	57.8	319 bp	1.0	78.5
rpoB_p5	CCAAGCGGCTCTAGATTCAG	GCTCGCTAATGAGTACTGCA	55.0	50.0	58.1	57.8	320 bp	1.0	78.5