Hotspot

rpl2

IRb · 83298-84784 · 1487 bp

Back to Paracalia

Pi 0.00269

MarkerSeek score 60.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRb
length_bp: 1487
variable_sites: 4
pi: 0.00269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GAACGTGTCACAGCTTCCTC	CGCTTCAACCGGGTTATTCT	55.0	50.0	58.9	58.3	1619 bp	1.0	71.2
rpl2_p2	GAACGTGTCACAGCTTCCTC	ATCATTACGCTTCAACCGGG	55.0	50.0	58.9	58.3	1626 bp	1.0	70.6
rpl2_p3	TGAACGTGTCACAGCTTCC	CGCTTCAACCGGGTTATTCT	52.6	50.0	58.0	58.3	1620 bp	1.0	70.0
rpl2_p4	TGAACGTGTCACAGCTTCC	ATCATTACGCTTCAACCGGG	52.6	50.0	58.0	58.3	1627 bp	1.0	69.4
rpl2_p5	CGTGTCACAGCTTCCTCCTA	CGCTTCAACCGGGTTATTCT	55.0	50.0	59.1	58.3	1616 bp	1.0	69.2