Hotspot

ndhK

LSC · 49045-49722 · 678 bp

Back to Paracalia

Pi 0.00442

MarkerSeek score 61.30

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 3
pi: 0.00442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TCTGAAGATTGAGCCACAACT	GTTTATGCATGGCGAAAGGG	42.9	50.0	57.2	57.8	817-818 bp	1.0	64.5
ndhK_p2	CTGTGCAAAAAGGAATCCTGT	GTTTATGCATGGCGAAAGGG	42.9	50.0	57.0	57.8	789-790 bp	1.0	62.6
ndhK_p3	TCTGAAGATTGAGCCACAACT	GGGGCATTGGAATGGTCTTA	42.9	50.0	57.2	57.5	799-800 bp	1.0	62.6
ndhK_p4	TCTGAAGATTGAGCCACAACTT	GTTTATGCATGGCGAAAGGG	40.9	50.0	57.9	57.8	817-818 bp	1.0	62.0
ndhK_p5	TTCTGAAGATTGAGCCACAACT	GTTTATGCATGGCGAAAGGG	40.9	50.0	57.9	57.8	818-819 bp	1.0	62.0