Hotspot

ycf15-trnV-GAC

IRb · 98145-98824 · 680 bp

Back to Paracalia

Pi 0.00294
MarkerSeek score 60.40
Primer available yes
Label ycf15-trnV-GAC

Feature Metadata

label_name
ycf15-trnV-GAC
feature_type
igs
region
IRb
length_bp
680
variable_sites
2
pi
0.00294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnV-GAC_p1 ACCGTGAGAAAAGCAAGGAG GATGACTTCCACCACGTCAA 50.0 50.0 58.1 57.8 810 bp 1.0 78.0
ycf15-trnV-GAC_p2 CGTGAGAAAAGCAAGGAGGT GATGACTTCCACCACGTCAA 50.0 50.0 58.1 57.8 808 bp 1.0 78.0
ycf15-trnV-GAC_p3 ACCGTGAGAAAAGCAAGGAG AACTCGCATTGGGTTTAGGG 50.0 50.0 58.1 58.2 847 bp 1.0 77.9
ycf15-trnV-GAC_p4 CGTGAGAAAAGCAAGGAGGT AACTCGCATTGGGTTTAGGG 50.0 50.0 58.1 58.2 845 bp 1.0 77.9
ycf15-trnV-GAC_p5 CGTGAGAAAAGCAAGGAGGT ATGACTTCCACCACGTCAAG 50.0 50.0 58.1 57.8 807 bp 1.0 77.9