Hotspot

rbcL-accD

LSC · 56087-56607 · 521 bp

Back to Paracalia

Pi 0.00768

MarkerSeek score 61.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 4
pi: 0.00768
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGGCTACCAAATGGAGTCCT	AGCCTACATCTGTGTTCGAAC	50.0	47.6	58.0	58.0	753 bp	1.0	71.9
rbcL-accD_p2	AGGCTACCAAATGGAGTCCT	GCCTACATCTGTGTTCGAACT	50.0	47.6	58.0	58.0	752 bp	1.0	71.9
rbcL-accD_p3	GGCTACCAAATGGAGTCCTG	AGCCTACATCTGTGTTCGAAC	55.0	47.6	58.0	58.0	752 bp	1.0	71.8
rbcL-accD_p4	GGCTACCAAATGGAGTCCTG	GCCTACATCTGTGTTCGAACT	55.0	47.6	58.0	58.0	751 bp	1.0	71.8
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	GCCTACATCTGTGTTCGAACT	55.0	47.6	57.7	58.0	739 bp	1.0	69.8