Hotspot

trnM-CAT-atpE

LSC · 51792-51998 · 207 bp

Back to Paracalia

Pi 0.0386

MarkerSeek score 70.10

Primer available yes

Label trnM-CAT-atpE

Feature Metadata

label_name: trnM-CAT-atpE
feature_type: igs
region: LSC
length_bp: 207
variable_sites: 8
pi: 0.0386
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAT-atpE_p1	GCGGGAGTCATTGGTTCAAA	CAAATCTAGCTCTCCGACGG	50.0	55.0	58.8	57.9	300 bp	1.0	75.1
trnM-CAT-atpE_p2	GCGGGAGTCATTGGTTCAAA	GGCAAATCTAGCTCTCCGAC	50.0	55.0	58.8	58.1	302 bp	1.0	74.9
trnM-CAT-atpE_p3	GCGGGAGTCATTGGTTCAAA	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.8	57.9	304 bp	1.0	74.9
trnM-CAT-atpE_p4	GCGGGAGTCATTGGTTCAAA	AGGCAAATCTAGCTCTCCGA	50.0	50.0	58.8	58.2	303 bp	1.0	74.3
trnM-CAT-atpE_p5	GCGGGAGTCATTGGTTCAAA	CTAGGACACGAGTAGAGGCT	50.0	55.0	58.8	57.7	279 bp	1.0	73.6