Hotspot

rps16-trnQ-TTG

LSC · 6346-7322 · 977 bp

Back to Paracalia

Pi 0.00309

MarkerSeek score 61.20

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 977
variable_sites: 3
pi: 0.00309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1043-1050 bp	1.0	79.0
rps16-trnQ-TTG_p2	AGAATAAGAATCCACGCCGG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1075-1082 bp	1.0	78.2
rps16-trnQ-TTG_p3	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1058-1065 bp	1.0	77.2
rps16-trnQ-TTG_p4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1057-1064 bp	1.0	77.2
rps16-trnQ-TTG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1047-1054 bp	1.0	74.3