Hotspot

ycf1

SSC · 109256-114724 · 5469 bp

Back to Paraboea

Pi 0.0214

MarkerSeek score 58.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5469
variable_sites: 313
pi: 0.0214
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGGCCCTCTTATCTCTTCC	CGATTCCATCGCTGCAAATC	55.0	50.0	57.9	58.0	1002-1014 bp	1.0	85.9
ycf1_p2	GGGCCCTCTTATCTCTTCCT	CGATTCCATCGCTGCAAATC	55.0	50.0	57.9	58.0	1001-1013 bp	1.0	85.9
ycf1_p3	TCCGAGCTCAGGTTATGGAA	CGATTCCATCGCTGCAAATC	50.0	50.0	58.1	58.0	979-991 bp	1.0	85.7
ycf1_p4	ACGGAAGAAAGGCTGGAAAG	CGATTCCATCGCTGCAAATC	50.0	50.0	58.1	58.0	368-377 bp	0.857143	83.7
ycf1_p5	CGGAAGAAAGGCTGGAAAGT	CGATTCCATCGCTGCAAATC	50.0	50.0	58.1	58.0	367-376 bp	0.857143	83.7