Hotspot

trnL-UAG-rpl32

SSC · 124232-125093 · 862 bp

Back to Paraboea

Pi 0.0369
MarkerSeek score 76.20
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
862
variable_sites
79
pi
0.0369
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TCCTAAGAGCAGCGTGTCTA ATCGTTCCCCTATACCGGTT 50.0 50.0 57.9 57.9 1235-1260 bp 0.857143 81.1
trnL-UAG-rpl32_p2 TTTAGGAGATGACATGCCGC ATCGTTCCCCTATACCGGTT 50.0 50.0 58.0 57.9 1290-1304 bp 0.714286 75.3
trnL-UAG-rpl32_p3 TTTAGGAGATGACATGCCGC TCGGCGTATTTTCCCGAATT 50.0 45.0 58.0 58.0 1569-1586 bp 0.428571 59.2
trnL-UAG-rpl32_p4 TCCTAAGAGCAGCGTGTCTA TCGGCGTATTTTCCCGAATT 50.0 45.0 57.9 58.0 1514-1531 bp 0.428571 58.7
trnL-UAG-rpl32_p5 AGCAGCGTGTCTACCAATTT TCGGCGTATTTTCCCGAATT 45.0 45.0 57.8 58.0 1507-1524 bp 0.428571 58.2