Hotspot

psaC-ndhD

SSC · 121233-121333 · 101 bp

Back to Paraboea

Pi 0.0682

MarkerSeek score 73.20

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 101
variable_sites: 14
pi: 0.0682
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	ACTCGTAGTATGGGTCTAGCT	TCGTGGTAAAGACAAGATACA	47.6	38.1	57.1	54.4	139-150 bp	1.0	51.7
psaC-ndhD_p2	ACTCGTAGTATGGGTCTAGCTT	TCGTGGTAAAGACAAGATACA	45.5	38.1	57.8	54.4	139-150 bp	1.0	51.7
psaC-ndhD_p3	ACTCGTAGTATGGGTCTAGCT	TCGTGGTAAAGACAAGATACAT	47.6	36.4	57.1	54.9	139-150 bp	1.0	51.7
psaC-ndhD_p4	ACTCGTAGTATGGGTCTAGCT	TTCGTGGTAAAGACAAGATACA	47.6	36.4	57.1	55.2	140-151 bp	1.0	51.6
psaC-ndhD_p5	AACTCGTAGTATGGGTCTAGCT	TCGTGGTAAAGACAAGATACA	45.5	38.1	57.8	54.4	140-151 bp	1.0	51.6