Hotspot

psaI-ycf4

LSC · 60433-60860 · 428 bp

Back to Paraboea

Pi 0.0296

MarkerSeek score 70.60

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 30
pi: 0.0296
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	ATAAAGAAACCCGCGATCCC	45.0	50.0	58.0	58.0	695-722 bp	1.0	86.6
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	TTCCAGGAAATTGTGCACCA	45.0	45.0	58.0	57.9	728-755 bp	1.0	85.9
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	TGCATCGATTTGGATACGCA	45.0	45.0	58.0	58.1	1210-1231 bp	1.0	85.7
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	AATCGGAGGAATATGCGACG	45.0	50.0	58.0	57.9	827-854 bp	1.0	85.5
psaI-ycf4_p5	ATTTCCGGCAATTGCAATGG	CGGAGGAATATGCGACGATT	45.0	50.0	58.0	57.9	824-851 bp	1.0	85.5