Hotspot

trnM-CAU-atpE

LSC · 53126-53328 · 203 bp

Back to Paraboea

Pi 0.0629

MarkerSeek score 76.10

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 30
pi: 0.0629
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	ATGGTGGGGTCTCTTTGGTA	AAGAGCGCGAAAAATTGAGC	50.0	45.0	57.9	58.0	1257-1268 bp	1.0	83.2
trnM-CAU-atpE_p2	CCCAAATCGTACCGAACCAT	AAGAGCGCGAAAAATTGAGC	50.0	45.0	58.0	58.0	1228 bp	0.428571	57.3
trnM-CAU-atpE_p3	CCCAAATCGTACCGAACCAT	CGGGCTAGGACAAGAGTAGA	50.0	55.0	58.0	57.9	640 bp	0.428571	57.3
trnM-CAU-atpE_p4	TGATCCAGGAGCACTACCAA	CGGGCTAGGACAAGAGTAGA	50.0	55.0	58.0	57.9	916-917 bp	0.428571	57.3
trnM-CAU-atpE_p5	TGATCCAGGAGCACTACCAA	AAGAGCGCGAAAAATTGAGC	50.0	45.0	58.0	58.0	1504-1505 bp	0.428571	57.2