Hotspot

trnF-GAA-ndhJ

LSC · 48812-49499 · 688 bp

Back to Paraboea

Pi 0.0312

MarkerSeek score 69.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 51
pi: 0.0312
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AAAGATGCAGGGTCGTTTGT	55.0	45.0	58.0	58.0	1229-1242 bp	0.857143	79.1
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	TTGTCTGTTTGGCTCGTGAA	55.0	45.0	58.0	58.0	1213-1226 bp	0.857143	78.3
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TTTGTCTGTTTGGCTCGTGA	55.0	45.0	58.0	58.0	1214-1227 bp	0.857143	78.3
trnF-GAA-ndhJ_p4	CTACATTGGGAATGGTCGGG	AAAGATGCAGGGTCGTTTGT	55.0	45.0	58.0	58.0	1246-1259 bp	0.714286	72.3
trnF-GAA-ndhJ_p5	TGCTACATTGGGAATGGTCG	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.0	58.0	1248-1261 bp	0.714286	72.3