Hotspot

petN-psbM

LSC · 29198-30096 · 899 bp

Back to Paraboea

Pi 0.019

MarkerSeek score 71.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 52
pi: 0.019
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCGTTTCTAC	50.0	50.0	58.0	57.4	976-1091 bp	1.0	81.9
petN-psbM_p2	ACTCGTAGTGTGGGGAAGAA	GTTCCTACCGCGTTTCTACT	50.0	50.0	58.0	57.4	975-1090 bp	1.0	81.9
petN-psbM_p3	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCGTTTCTAC	50.0	50.0	58.0	57.4	974-1089 bp	1.0	81.9
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	GTTCCTACCGCGTTTCTACT	50.0	50.0	58.0	57.4	973-1088 bp	1.0	81.9
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	GTTCCTACCGCGTTTCTACT	55.0	50.0	57.9	57.4	972-1087 bp	1.0	81.3