Hotspot

psbI-trnS-GCU

LSC · 8366-8486 · 121 bp

Back to Paraboea

Pi 0.0415

MarkerSeek score 77.00

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 12
pi: 0.0415
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	157-190 bp	1.0	78.6
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGAA	CGAGTTATTCGTACCGAGGG	47.6	55.0	57.8	57.6	157-190 bp	1.0	76.3
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	TTATTCGTACCGAGGGTTCG	50.0	50.0	57.1	57.2	153-186 bp	1.0	75.1
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGA	TTCGAATCCCTCTCTTTCCG	50.0	50.0	57.1	57.0	137-170 bp	1.0	74.8
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	GGGTTCGAATCCCTCTCTTT	50.0	50.0	57.1	56.9	140-173 bp	1.0	73.7