Hotspot

rps16-trnQ-UUG

LSC · 6177-7244 · 1068 bp

Back to Paraboea

Pi 0.0258
MarkerSeek score 68.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1068
variable_sites
76
pi
0.0258
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.95
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGATGTCGAGACAAGAGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1212-1238 bp 1.0 88.7
rps16-trnQ-UUG_p2 AACGATGTCGAGACAAGAGC AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.0 58.1 1772-1798 bp 1.0 88.4
rps16-trnQ-UUG_p3 AACGATGTCGAGACAAGAGC CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 1777-1803 bp 1.0 88.4
rps16-trnQ-UUG_p4 AACGATGTCGAGACAAGAGC GTGCGAATCGAGGGTTCATA 50.0 50.0 58.0 57.8 1920-2018 bp 1.0 87.1
rps16-trnQ-UUG_p5 AACGATGTCGAGACAAGAGC TGCGAATCGAGGGTTCATAC 50.0 50.0 58.0 57.8 1919-2017 bp 1.0 87.1