Hotspot

ndhG-rps15

SSC · 115842-116657 · 816 bp

Back to Papilionanthe

Pi 0.0196
MarkerSeek score 59.70
Primer available yes
Label ndhG-rps15

Feature Metadata

label_name
ndhG-rps15
feature_type
igs
region
SSC
length_bp
816
variable_sites
15
pi
0.0196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-rps15_p1 AGTGTTCTAGCCATGCTTCG ATCACAAAGGGGTCTACGAA 50.0 45.0 58.0 56.2 1126-1171 bp 1.0 70.0
ndhG-rps15_p2 AGTGTTCTAGCCATGCTTCG TCACAAAGGGGTCTACGAAT 50.0 45.0 58.0 56.2 1125-1170 bp 1.0 70.0
ndhG-rps15_p3 AGTGTTCTAGCCATGCTTCG ATCACAAAGGGGTCTACGAAT 50.0 42.9 58.0 56.7 1126-1171 bp 1.0 66.4
ndhG-rps15_p4 CGGCTTGTGATCAGTCCATA ATCACAAAGGGGTCTACGAA 50.0 45.0 57.4 56.2 1108-1153 bp 1.0 65.4
ndhG-rps15_p5 CGGCTTGTGATCAGTCCATA TCACAAAGGGGTCTACGAAT 50.0 45.0 57.4 56.2 1107-1152 bp 1.0 65.4