Hotspot

ndhD-psaC

SSC · 114383-114521 · 139 bp

Back to Papilionanthe

Pi 0.0288

MarkerSeek score 66.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 139
variable_sites: 4
pi: 0.0288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAACTCGCAAATATGGGCA	AGATGTGAATCCGCTTGTCC	45.0	50.0	57.6	58.0	279 bp	1.0	77.6
ndhD-psaC_p2	AGAACTCGCAAATATGGGCA	ATGTGAATCCGCTTGTCCAA	45.0	45.0	57.6	57.8	277 bp	1.0	76.2
ndhD-psaC_p3	AGAACTCGCAAATATGGGCA	CGCAGCATGGGTCTTTCTTA	45.0	50.0	57.6	58.3	213 bp	1.0	76.1
ndhD-psaC_p4	AGAACTCGCAAATATGGGCA	GATGTGAATCCGCTTGTCCA	45.0	50.0	57.6	58.3	278 bp	1.0	75.7
ndhD-psaC_p5	AGAACTCGCAAATATGGGCA	CGCTTGTCCAACGGATTTTT	45.0	45.0	57.6	57.6	268 bp	1.0	74.5