Hotspot

trnN-GUU-ndhI

IRb · 110953-111369 · 417 bp

Back to Papilionanthe

Pi 0.0239

MarkerSeek score 59.00

Primer available yes

Label trnN-GUU-ndhI

Feature Metadata

label_name: trnN-GUU-ndhI
feature_type: igs
region: IRb
length_bp: 417
variable_sites: 9
pi: 0.0239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhI_p1	AAAGCGAGCCCTTTCATTCA	TGGAACTGCCATTCAAAAGGT	45.0	42.9	58.1	58.3	896-1025 bp	1.0	74.1
trnN-GUU-ndhI_p2	AAGCGAGCCCTTTCATTCAA	TGGAACTGCCATTCAAAAGGT	45.0	42.9	58.1	58.3	895-1024 bp	1.0	74.1
trnN-GUU-ndhI_p3	TCAAAGCGAGCCCTTTCATT	TGGAACTGCCATTCAAAAGGT	45.0	42.9	58.1	58.3	898-1027 bp	1.0	74.1
trnN-GUU-ndhI_p4	TATGCCGAGGTATTGAGGGT	TGGAACTGCCATTCAAAAGGT	50.0	42.9	57.9	58.3	1008-1137 bp	1.0	73.5
trnN-GUU-ndhI_p5	GGCAGAATAGCAGAGCAAGT	TGGAACTGCCATTCAAAAGGT	50.0	42.9	58.0	58.3	1172-1301 bp	1.0	73.3