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clpP-psbB

LSC · 73223-73954 · 732 bp

Back to Papilionanthe

Pi 0.0418

MarkerSeek score 70.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 30
pi: 0.0418
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	58.0	58.0	906-916 bp	1.0	83.7
clpP-psbB_p2	CGCACTATACGTCAACCCAA	AAGCAAGCGATTCCTGAGAG	50.0	50.0	58.0	58.0	1260-1270 bp	1.0	82.7
clpP-psbB_p3	CGCACTATACGTCAACCCAA	TAGACCCGCTAATATGCCCA	50.0	50.0	58.0	58.0	1450-1460 bp	1.0	82.4
clpP-psbB_p4	TGGATTGGGCGAAACAAAGA	AAGCAAGCGATTCCTGAGAG	45.0	50.0	58.0	58.0	1337-1347 bp	1.0	82.4
clpP-psbB_p5	TGGATTGGGCGAAACAAAGA	TAGACCCGCTAATATGCCCA	45.0	50.0	58.0	58.0	1527-1537 bp	1.0	82.2