Hotspot

petA-psbJ

LSC · 63813-64798 · 986 bp

Back to Papilionanthe

Pi 0.0185

MarkerSeek score 61.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 986
variable_sites: 18
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	GGATCTTATTCCGGGTTGGG	50.0	55.0	58.0	57.7	1093-1104 bp	1.0	79.9
petA-psbJ_p2	GAGTCCAAGGCCTTCTGTTC	GGATCTTATTCCGGGTTGGG	55.0	55.0	58.2	57.7	1118-1129 bp	1.0	78.4
petA-psbJ_p3	GGCATCTGTGATTTTGGCAC	CTTATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.5	1089-1100 bp	1.0	78.1
petA-psbJ_p4	GTCCAAGGCCTTCTGTTCTT	GGATCTTATTCCGGGTTGGG	50.0	55.0	57.7	57.7	1116-1127 bp	1.0	77.7
petA-psbJ_p5	TTACGAGTCCAAGGCCTTCT	GGATCTTATTCCGGGTTGGG	50.0	55.0	58.4	57.7	1122-1133 bp	1.0	77.0