Hotspot

atpH-atpI

LSC · 14552-15234 · 683 bp

Back to Papilionanthe

Pi 0.00614

MarkerSeek score 66.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 4
pi: 0.00614
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.9	57.9	772-801 bp	1.0	82.0
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.1	57.9	823-852 bp	1.0	81.9
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	751-780 bp	1.0	81.6
atpH-atpI_p4	CCGATAGAAGCAAGCCCTAC	AACTTTGGCTGCGGCTTATA	55.0	45.0	57.8	57.9	771-800 bp	1.0	81.4
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	750-779 bp	1.0	81.1