Hotspot

ndhD-trnL-UAG

IRb · 119602-120869 · 1268 bp

Back to Paphiopedilum

Pi 0.0531

MarkerSeek score 78.80

Primer available yes

Label ndhD-trnL-UAG

Feature Metadata

label_name: ndhD-trnL-UAG
feature_type: igs
region: IRb
length_bp: 1268
variable_sites: 553
pi: 0.0531
unique_haplotype_count: 63
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-trnL-UAG_p1	GATCTGTATCTTTCTACCTGTAA	TCCTAAGAGCAGCGTGTCTA	34.8	50.0	52.8	57.9	1338-2281 bp	0.746269	41.9
ndhD-trnL-UAG_p2	GATCTGTATCTTTCTACCTGTAA	AGCAGCGTGTCTACCAATTT	34.8	45.0	52.8	57.8	1331-2274 bp	0.746269	41.9
ndhD-trnL-UAG_p3	GATCTGTATCTTTCTACCTGTAA	CCTAAGAGCAGCGTGTCTAC	34.8	55.0	52.8	57.8	1337-2280 bp	0.746269	41.9
ndhD-trnL-UAG_p4	GATCTGTATCTTTCTACCTGTAA	CTAAGAGCAGCGTGTCTACC	34.8	55.0	52.8	57.8	1336-2279 bp	0.746269	41.9
ndhD-trnL-UAG_p5	GATCTGTATCTTTCTACCTGTAA	GAGCAGCGTGTCTACCAATT	34.8	50.0	52.8	58.3	1332-2275 bp	0.746269	41.9