Hotspot

rps8-rpl14

LSC · 82297-82743 · 447 bp

Back to Paphiopedilum

Pi 0.0469

MarkerSeek score 85.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 136
pi: 0.0469
unique_haplotype_count: 59
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGGCAATAATGTCCCTACCC	TTGCTCGGGAATTGAGACAG	55.0	50.0	58.1	57.9	485-739 bp	0.985075	89.7
rps8-rpl14_p2	CGGCAATAATGTCCCTACCC	GCTCGGGAATTGAGACAGTT	55.0	50.0	58.1	57.9	483-737 bp	0.985075	89.7
rps8-rpl14_p3	CGGCAATAATGTCCCTACCC	CGATTGCTCGGGAATTGAGA	55.0	50.0	58.1	58.1	488-742 bp	0.970149	89.5
rps8-rpl14_p4	CGGCAATAATGTCCCTACCC	ATTGCTCGGGAATTGAGACA	55.0	45.0	58.1	57.2	486-740 bp	0.985075	84.2
rps8-rpl14_p5	TCGGCAATAATGTCCCTACC	CGATTGCTCGGGAATTGAGA	50.0	50.0	57.1	58.1	489-743 bp	0.970149	82.9