Hotspot

psbB-psbT

LSC · 75424-75850 · 427 bp

Back to Paphiopedilum

Pi 0.109

MarkerSeek score 78.00

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 214
pi: 0.109
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	376-834 bp	0.985075	75.1
psbB-psbT_p2	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	375-833 bp	0.985075	72.9
psbB-psbT_p3	TTTGCCGGTATTGATCCAGA	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	56.9	57.6	403-861 bp	1.0	72.1
psbB-psbT_p4	TGCCGGTATTGATCCAGATT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	56.7	57.6	401-859 bp	1.0	70.2
psbB-psbT_p5	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGAA	50.0	45.5	57.4	58.0	375-833 bp	0.985075	70.2