Hotspot

rpl33-rps18

LSC · 68266-68621 · 356 bp

Back to Paphiopedilum

Pi 0.0655

MarkerSeek score 75.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 138
pi: 0.0655
unique_haplotype_count: 61
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TGATTCATGGGGAAATAAAGA	TAATCGATTCGATCCCCCGA	33.3	50.0	52.4	58.1	335-680 bp	0.985075	51.4
rpl33-rps18_p2	TGATTCATGGGGAAATAAAGAA	TAATCGATTCGATCCCCCGA	31.8	50.0	53.4	58.1	335-680 bp	0.985075	51.4
rpl33-rps18_p3	TGATTCATGGGGAAATAAAGA	GGGGTAAACGCCTACGAAAA	33.3	50.0	52.4	58.2	307-652 bp	0.985075	51.4
rpl33-rps18_p4	TGATTCATGGGGAAATAAAGAA	GGGGTAAACGCCTACGAAAA	31.8	50.0	53.4	58.2	307-652 bp	0.985075	51.4
rpl33-rps18_p5	TGATTCATGGGGAAATAAAGAAA	TAATCGATTCGATCCCCCGA	30.4	50.0	54.2	58.1	335-680 bp	0.985075	51.4