Hotspot

petN-psbM

LSC · 29107-30285 · 1179 bp

Back to Paphiopedilum

Pi 0.066

MarkerSeek score 89.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 495
pi: 0.066
unique_haplotype_count: 63
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.2	57.9	617-1858 bp	0.985075	89.6
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	612-1853 bp	1.0	88.7
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	613-1854 bp	1.0	87.8
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGCTGTTCATTT	50.0	45.0	58.2	58.5	610-1851 bp	1.0	87.0
petN-psbM_p5	TAGTATGGGGAAGGAGTGGG	CGTTTATTGCTACTGCGCTG	55.0	50.0	57.5	57.9	573-1801 bp	0.970149	86.3